Enzyme and protein composition of myelin isolated in the presence of EGTA |
| |
Authors: | Philippe Burgisser Jean-Marie Matthieu |
| |
Affiliation: | Laboratoire de Neurochimie, Service de Pédiatrie, Centre Hospitalier Universitaire Vaudois, CH-1011 Lausanne, Switzerland |
| |
Abstract: | The efficiency of ethyleneglycol-bis (β-amino-ethyl ether) N,N′-tetra-acetic acid (EGTA) in removing possible contamination from myelin was tested. Myelin fractions were isolated in the presence or absence of EGTA. An axolemma-enriched fraction was also prepared. Gel electrophoresis showed no important alteration of the protein pattern of myelin treated with EGTA. Only a minor band of about 41,000 daltons was selectively removed when EGTA was used during the two density gradient and differential centrifugation steps. EGTA, when used in the final washes, did not remove this band. It was absent from axolemma-enriched fractions. Different hypotheses are considered to explain these findings. |
| |
Keywords: | To whom all correspondence should be sent. |
本文献已被 ScienceDirect 等数据库收录! |