Cysteine proteinase 30 in clinical isolates of T. vaginalis from symptomatic and asymptomatic infected women |
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Authors: | Yadav Manisha Dubey M L Gupta Indu Bhatti Gurjeet Malla Nancy |
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Institution: | Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India. |
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Abstract: | A cysteine proteinase of 30 kDa (CP30) of Trichomonas vaginalis, is known to play a role in cytoadherence of the parasite to host cells. However, the CP30 activity in clinical isolates from symptomatic and asymptomatic patients has not been analyzed. In the present study, CP30 was detected in 20 fresh and long-term culture maintained T. vaginalis isolates each from symptomatic and asymptomatic women by substrate gel electrophoresis and immunoblotting. Though CP30 was detected in all the fresh isolates from 20 symptomatic and 20 asymptomatic women, the intensity of CP30 band was significantly higher in isolates from symptomatic as compared to asymptomatic women indicating higher expression in former. CP30 was found in all the 20 long-term cultured isolates from symptomatic whereas only in 70% of asymptomatic women indicating that CP30 expression is a more stable characteristic of symptomatic isolates. The isolates from symptomatic women, demonstrated significantly higher cytoadherence to VECs as compared to asymptomatic women. In both the types of isolates, this cytoadherence was inhibited significantly by CP30 specific hyperimmune serum. These results confirm that CP30 is an important virulence factor of T. vaginalis and has an important role in cytoadherence to VECs and thus has a role in pathogenesis of trichomoniasis. |
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Keywords: | Trichomonas vaginalis Cytoadherence Trichomoniasis CP30 cysteine proteinase 30 c p m count per minute E-64 l-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane" target="_blank">l-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane HIS hyperimmune serum NRS normal rabbit serum VECs vaginal epithelial cells |
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