飞机草花蕾中Beclin1基因的克隆与表达

以入侵植物飞机草的不同时期花蕾为材料,利用RT-PCR法扩增得到了与PCD相关的类Beclin1基因的部分cDNA序列(大约700 bp),与烟草叶片中的基因序列(AY701316)同源性为95%;Northern blotting结果表明类Beclin1基因在花蕾发育中期的表达量高于初期和后期;通过DNA ladder检测表明在花蕾发育过程中伴随着PCD的发生,这些结果表明花蕾发育中期是PCD初期发生的活跃期,是绒毡层逐渐退化和花粉不断形成的过程.通过对生殖过程中的细胞程序性死亡的分子生物学研究,初步揭示了飞机草入侵过程与PCD的相互关系.

Cloning and Characterization of Beclin1 -like Gene in Eupatorium odoratum L. Buds

Beclin1 -like gene was cloned from three different growth periods flower buds of Eupatorium odoratum by RT-PCR method in this study. The homology between Beclin1 -like gene (about 700 bp)and Beclin1 gene in tobacco leaves is 95 %; Northern blotting result showed that the expression of Beclin 1 -like gene in the middle stage was higher than those in initial stage and dernier stage; the result of DNA ladder showed Programmed Cell Death (PCD) happened in the developing process of flower buds.All the results proved that PCD certainly happened with the tapetum degenerating and the pollen coming into being, which indicated the relationship between the aggressive process of E. odoratum and PCD in the process of the reproduction.