Isolation of uncultivable anaerobic thermophiles of the family Clostridiaceae requiring growth-supporting factors |
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Authors: | Kim Joong-Jae Kim Hee Na Masui Ryoji Kuramitsu Seili Seo Jin-Ho Kim Kwang Sung Moon-Hee |
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Affiliation: | Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan. |
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Abstract: | Novel groups of uncultivable anaerobic thermophiles were isolated from compost by enrichment cultivation in medium with a cell-free extract of Geobacillus toebii. The cell-free extract of G. toebii provided the medium with growth-supporting factors (GSF) needed to cultivate the previously uncultured microorganisms. Twenty-nine GSF-requiring candidates were successfully cultivated, and 16 isolated novel bacterial strains were classified into three different groups of uncultivable bacteria. The similarity among these 16 isolates and a phylogenetic analysis using 16S rRNA gene sequences revealed that these GSF-requiring strains represented novel groups within the family Clostridiaceae. |
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