黄连体细胞胚胎发生的研究

黄连(Coptis chinensis)叶片外植体在 MS 2,4-D 1 ppm 培养基上很容易产生愈伤组织。愈伤组织在转入分化培养基 MS 6-BA 0.5ppm NAA 1ppm 培养基上以后,能产生大量胚状体。胚状体可经过球形、心形、鱼雷形及子叶期等诸阶段发育成小植株。对胚状体用4%的藻酸钠和2%的氯化钙进行人工种皮包埋后,在无菌条件下,胚状体转变成苗。愈伤组织在分化培养基上经几次继代后,整个愈伤组织可转变为胚性愈伤组织并形成一个个胚性细胞团。胚状体可从其表面或愈伤组织内的任一细胞团产生。这一研究结果为获得大量分散的单个胚状体及人工种子的研制提供了良好的实验系统。

STUDIES ON SOMATIC EMBRYOGENESIS OF COPTIS CHINENSIS

Leaf explants of Coptis chinensis were cultured on medium MS 2,4-D lppm and calli were easily induced.After the calli were transferred on to the medium MS 6-BA 0.5ppm NAA Ippm for differentiation,a lot of somatic embryos emerged.Embryoids developed into plantlets throughout processes of globular,heart-shaped,torpedo-shaped,and cotyledon stages. Embryoids were encapsulated in 4% sodium alginate and 2% CaCl_2 as artificial seed,then so- me somatic embryos encased in capsule converted to plantlets in the aseptic conditions.After the calli were subcultured on the medium through 3-4 passages for differentiation,the whole callus could be converted to embryogenic callus and formed numerous scattered embryogenic cell mass.The somatic embryos of C.chinensis may be produced from surface cells or any embryogenic cell mass of calli.These results could provide an excellent experimantal system for large scale to obtain single embryoids as well as making artificial seeds.