| 水稻叶绿体RNA聚合酶α亚基基因的克隆及结构分析 |
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| 引用本文： | 孙崇荣 杉浦昌弘. 水稻叶绿体RNA聚合酶α亚基基因的克隆及结构分析[J]. Acta Botanica Sinica, 1989, 31(10): 763-769 |
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| 作者姓名： | 孙崇荣 杉浦昌弘 |
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| 作者单位： | 复旦大学生化系 上海200433(孙崇荣)，名古屋大学基因研究中心 日本(杉浦昌弘) |
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| 摘 要： | Southern 印迹杂交结果表明,水稻叶绿体基因组也能编码自身的 RNA 聚合酶。该酶的α基基因片段已经被克隆于大肠杆菌质粒 pUC 19中。应用逐次丢失法和双脱氧方法测定了它的核苷酸排列顺序。该基因由337个密码子组成。在单子叶的水稻和双子叶的烟草之间该基因有很高的同源性。
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| 关 键 词： | 水稻叶绿体 RNA 聚合酶 rpoA 基因 |
CLONING AND SEQUENCING OF rpoA GENE FROM RICE CHLOROPLAST GENOME |
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| Sun Chong-rong. CLONING AND SEQUENCING OF rpoA GENE FROM RICE CHLOROPLAST GENOME[J]. , 1989, 31(10): 763-769 |
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| Authors: | Sun Chong-rong |
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| Abstract: | The result of southern hybridization showed that the rice chloroplast genome can encode its own RNA polymerase.The a subunit gene fragment of this enzyme has been cloned into E.coli plasmid pUC 19.By deletion method and dideoxynucleotide chain termination,we analysed its nucleotides sequence.This gene consists of 337 codons.It reserves a high homo- logy between rice,monocotyledonous plant and tobacco,dicotyledonous. |
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| Keywords: | Rice chloroplast RNA polymerase rpo gene |
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