大麦G—显带核型的研究

本文报道了 ASG 法处理的三个栽培大麦(Hordeum Vulgare)品种 G-带的核型研究。结果表明无论是早中期或中期染色体都显示出了密切邻近的、多重的 G-带带纹。在有丝分裂过程中染色体愈浓缩带纹数目愈少。同源染色体之间带纹分布的位置、染色深浅以及带纹数目都基本一致,可以较为准确地进行配对。同一分裂时期不同染色体的 G-带带纹各具一定的特点,可以作为鉴别的标记。讨论了显带技术和中期染色体的 G-带等问题。

STUDIES ON G-BANDING KARYOTYPE IN BARLEY(HORDEUM VULGARE)

G-banding karyotypes of three cultivars in barley were analyzed.Multiple closely adja- cent G-bands were able to be observed in each early metaphase or metaphase chromosome treat- ted by an ASG method.The more concentrated the chromosome,the less was the number of G-bands during mitosis.The position of band distribution,staining degree and band numbers between homologous chromosomes were basically identical.Chromosome pairing for karyotype analysis could be carried out more accurately.G-banding patterns of different chromosome pairs were not the same,they could be used as the markers to distinguish one from another chromosome pair.During the same mitotic stage the banding patterns including number,rela- tive position and staining degree of the bands between different cultivars were basically the same,but they had differences in the size and staining degree of some bands near centromeres. G-banding technique and G-banding of metaphase chromosomes were discussed.