| 人参原生质体培养再生愈伤组织 |
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| 引用本文： | 李乐工. 人参原生质体培养再生愈伤组织[J]. Acta Botanica Sinica, 1989, 31(10): 815-816 |
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| 作者姓名： | 李乐工 |
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| 作者单位： | 中国医学科学院药物研究所 北京100050 |
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| 摘 要： | 人参原生质体培养未见报道成功。Harn(1974)曾用人参幼叶,幼根和上胚轴进行原生质体分离,但得到的数量很少,无法进行培养。本实验以人参培养细胞为材料,通过培养再生了愈伤组织。
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| 关 键 词： | 人参 原生质体培养 愈伤组织再生 |
CALLUS REGENERATION FROM PROTOPLASTS OF PANAX GINSENG |
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| Li Le-gong. CALLUS REGENERATION FROM PROTOPLASTS OF PANAX GINSENG[J]. , 1989, 31(10): 815-816 |
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| Authors: | Li Le-gong |
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| Abstract: | Protoplasts were enzymatically isolated from the cultured cell line No.246 of Panax ginseng and suspended in a 67V-D liquid medium.After 35 days of culture, the cell clusters derived from protoplasts were transfered to a 67V-E semisolid medium.When the cell clusters grew to 2mm approximately,they were transfered to common 67V medium for the callus regeneration. |
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| Keywords: | Panax ginseng Protoplast culture Callus regeneration |
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