| 1,3-丙二醇产生菌的诱变育种及培养基优化 |
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| 引用本文： | 王扬,方慧英,诸葛斌,诸葛健. 1,3-丙二醇产生菌的诱变育种及培养基优化[J]. 工业微生物, 2010, 40(4): 13-17. DOI: 10.3969/j.issn.1001-6678.2010.04.003 |
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| 作者姓名： | 王扬 方慧英 诸葛斌 诸葛健 |
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| 作者单位： | 江南大学,工业生物技术教育部重点实验室,工业微生物研究中心,江苏无锡,214122 |
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| 基金项目： | "863"国家科技攻关项目 |
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| 摘 要： | 以实验室自然筛选的克雷伯氏杆菌(Klebsiella sp.)为出发株,采用紫外诱变及亚硝基胍和超声波协同处理获得一株1,3-丙二醇高产突变株。在摇瓶发酵中,其产1,3-丙二醇产量由17.39 g/L提高到24.11 g/L,提高38.64%。变异株经10次传代培养,发酵能力稳定。对发酵培养基成分进行了优化,优化后1,3-丙二醇产量为30.05g/L,为优化前的1.25倍。
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| 关 键 词： | 克雷伯氏杆菌 1，3-丙二醇 诱变 培养基 |
Improvement of 1,3-propanediol-producing strains by mutation and optimization of its culture medium |
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| WANG Yang,FANG Hui-ying,ZHUG GE Bin,ZHUG GE Jian. Improvement of 1,3-propanediol-producing strains by mutation and optimization of its culture medium[J]. Industrial Microbiology, 2010, 40(4): 13-17. DOI: 10.3969/j.issn.1001-6678.2010.04.003 |
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| Authors: | WANG Yang FANG Hui-ying ZHUG GE Bin ZHUG GE Jian |
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| Affiliation: | WANG Yang, FANG Hui-ying, ZHUG GE Bin, ZHUG GE Jian (Key Laboratory of Industrial Biotechnology , Ministry of Education; Research Center of Industrial Microorganisms , Jiangnan University, Wuxi 214122, China) |
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| Abstract: | Klebsiella sp. screened from nature was used as the starting strain. A high 1,3-propanediol-producing strain was obtained by mutation with UV and NTG-ultrasound. Using the mutant strain, the production of 1,3-propanediol in flask fermentation was increased from 17.39 g/L to 24.11 g/L （by 38.64% ）. The mutant strain showed genetic stability during 10 generations. The fermentative culture medium compositions were optimized. The production of 1,3-propanediol using the optimum medium reached 30.05 g/L, which was 1.25 times than that of the basic medium. |
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| Keywords: | Klebsiella 1,3-propanediol mutation medium |
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