Pulmonary metabolism of prostacyclin (PGI2) in the rabbit. |
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Authors: | P Y Wong J C McGiff F F Sun K U Malik |
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Affiliation: | 1. Department of Pharmacology University of Tennessee Center for the Health Sciences Memphis, TN 38163 USA;2. The Upjohn Company Kalamazoo, Mich 49001 USA |
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Abstract: | Metabolism of prostacyclin, [9-3H]PGI2, was examined in the isolated perfused rabbit lung and the post-microsomal supernate of rabbit lung homogenate. Two major metabolites of [9-3H]PGI2 from the lung perfusate were separated by thin-layer chromatography and radiometric gas-chromatography. These two products were identified as 6 keto-PGF1α and 6,15 diketo-13,14 dihydro PGF1α by mass-spectrometry; they represented 65% and 14% of the total radioactivity. When [9-3H]PGI2 was incubated with the lung homogenate in the presence of either NAD+ or NADP+, more than 36% and 25%, respectively, was converted to the 6,15 diketo-13,14 dihydro metabolite. |
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Keywords: | To whom reprint request may be addressed |
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