Development of new biotin/streptavidin reagents for pretargeting |
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Authors: | Wilbur D S Pathare P M Hamlin D K Stayton P S To R Klumb L A Buhler K R Vessella R L |
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Affiliation: | Department of Radiation Oncology, University of Washington, Seattle 98195, USA. |
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Abstract: | The high affinity of biotin for streptavidin has made this pair of molecules very useful for in vivo applications. To optimize reagents for one potential in vivo application, antibody-based pretargeting of cancer, we have prepared a number of new biotin and streptavidin derivatives. The derivatives developed include new radiolabeled biotin reagents, new protein biotinylation reagents, and new biotin multimers for cross-linking and/or polymerization of streptavidin. We have also modified streptavidin by site-directed mutation and chemical modification to improve its in vivo characteristics, and have developed new reagents for cross-linking antibody fragments with streptavidin. A brief overview of these new reagents is provided. |
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