| Survivin靶向siRNA抑制大肠癌细胞增殖的作用 |
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| 引用本文： | 蔡明,王国斌,陶凯雄,蔡昌学. Survivin靶向siRNA抑制大肠癌细胞增殖的作用[J]. 中国组织化学与细胞化学杂志, 2008, 17(6): 519-522. DOI: 10.3870/zgzzhx.2008.06.001 |
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| 作者姓名： | 蔡明 王国斌 陶凯雄 蔡昌学 |
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| 作者单位： | 1. 华中科技大学同济医学院附属协和医院普外科,武汉,430022
2. 华中科技大学同济医学院病原生物系微生物学教研室,武汉,430030 |
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| 基金项目： | 国家863高技术研究发展计划资助项月 ， 湖北省科技攻关计划资助项目 |
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| 摘 要： | 目的 构建survivin靶向siRNA重组表达载体，研究其对人大肠癌细胞生长的抑制作用。方法构建Stirvivin靶向siRNA并转染结肠癌细胞，通过RT-PCR和Westernblot方法检测survivin的表达，采用MTT法检测siRNA对细胞增殖的抑制作用。结果经酶切鉴定和测序结果证实survivin靶向siRNA重组表达载体构建成功，它对大肠癌细胞survivinmRNA和蛋白的表达抑制率分别为36．33％和44．65％，肿瘤细胞的生长受到明显抑制。结论survivin靶向siRNA构建成功并能显著抑制survivin基因的表达。
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| 关 键 词： | Survivin RNA干扰 基因治疗 |
INHIBITING EFFECT OF THE SURVIVIN-TARGETTING siRNA RECOMBINANT EXPRESSION VECTOR ON HUMAN COLON CANCER CELL PROLIFERATION |
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| Cai Ming,Wang Guobin,Tao Kaixiong,Cai Changxue. INHIBITING EFFECT OF THE SURVIVIN-TARGETTING siRNA RECOMBINANT EXPRESSION VECTOR ON HUMAN COLON CANCER CELL PROLIFERATION[J]. Chinese Journal of Histochemistry and Cytochemistry, 2008, 17(6): 519-522. DOI: 10.3870/zgzzhx.2008.06.001 |
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| Authors: | Cai Ming Wang Guobin Tao Kaixiong Cai Changxue |
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| Affiliation: | Cai Ming1,Wang Guobin1,Tao Kaixiong1,Cai Changxue2(1Department of General Surgery,Union Hospital,Tongji Medical College,Huazhong Universityof Science , Technology,Wuhan 430022,2Department of Pathogenic Biology,School of Basic MedicalSciences,Huazhong University of Science , Technology,Wuhan 430030,China) |
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| Abstract: | Objective To construct the siRNA recombinant expression vector targetting survivin gene and to research its inhibiting effect on human colon cancer cell proliferation.Methods SiRNA targetting survivin gene was constructed and transfected into human colon cancer cells.The effect of siRNA was detected by RT-PCR,Western blot and MTT reduction assay.Results It was confirmed by restriction endonuclease and sequence analysis that siRNA recombinant expression vector targetting survivin gene was constructed success... |
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| Keywords: | Survivin |
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