Comparison of the intracellular lipids of cultured vascular endothelial cells and fibroblasts |
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Authors: | Edward J Macarak Margarita De la Llera Nicholas A Kefalides Barbara V Howard |
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Institution: | (1) Department of Biochemistry and Medicine, School of Medicine, University of Pennsylvania, Pennsylvania, USA;(2) Department of Biochemistry, Medical College of Pennsylvania, Pennsylvania, USA;(3) Connective Tissue Research Institute, University City Science Center, 3624 Market Street, 19104 Philadelphia, Pennsylvania;(4) Present address: Phoenix Clinical Research Section, NIAMDD, 4212 N. 16th Street, 85016 Phoenix, Arizona |
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Abstract: | Summary The lipids of human umbilical vein endothelial cells, calf aortic endothelial cells and foreskin fibroblasts have been compared.
Cell cultures were established, and, upon confluency, the lipids were extracted and analyzed with respect to total lipid content,
classes of lipids and total lipid fatty acid composition. The total quantity of lipid per milligram protein found in both
human umbilical vein endothelium and calf aorta endothelium was similar to that found in fibroblasts grown in similar medium.
Both types of endothelium contained the same major neutral lipid classes as fibroblasts, although they contained more phospholipid
than did fibroblasts. The fatty acid composition of the three cells examined was influenced by cell type as well as the type
of serum in the culture medium.
This work was supported by PHS Grants HL16058, HL19638, AM14626 and HL18827. |
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Keywords: | lipids fatty acids endothelial cells serum |
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