Photoacoustic spectroscopy of Anacystis nidulans. I. Effect of sample thickness on the photoacoustic signal |
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Authors: | R Carpentier B Larue R M Leblanc |
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Institution: | Centre de Recherche en Photobiophysique, Université du Québec à Trois-Rivières, Trois-Rivières, Québec G9A 5H7, Canada |
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Abstract: | A photoacoustic spectroscopy study of the cyanobacteria Anacystis nidulans has been undertaken. It is demonstrated, by using a filter deposition technique, that the photoacoustic signal intensity becomes progressively saturated as the thickness of the algal layer is increased. This saturation effect originates mostly from the limited optical penetration of the sample and distorts the photoacoustic spectrum from its true shape. A theoretical model is proposed to explain these results, and practical means to obviate the limitations of this spectroscopic technique are suggested. |
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