Biofunctional nanocomposite of carbon nanofiber with water-soluble porphyrin for highly sensitive ethanol biosensing |
| |
Authors: | Wu Lina Lei Jianping Zhang Xueji Ju Huangxian |
| |
Institution: | Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, PR China. |
| |
Abstract: | A biofunctional hybrid nanocomposite of carbon nanofiber (CNF) with water-soluble iron(III) meso-tetrakis(N-methylpyridinum-4-yl) porphyrin (FeTMPyP) was designed via non-covalent interaction for preparation of highly sensitive ethanol biosensor. The prepared nanocomposite showed good dispersion in water and was characterized with steady-state electronic absorption spectroscopy and scanning electron microscope. The nanocomposite combined the good conductivity of CNF and the excellent catalytic activity of both CNF and FeTMPyP toward the reduction of dissolved oxygen, producing a method for amperometric detection of oxygen ranging from 6.5 nM to 6.4 microM at a low overpotential. The nanocomposite modified electrode was further used for assembly of alcohol oxidase to construct an amperometric biosensor for ethanol. The biosensor showed rapid and highly sensitive response to ethanol with a linear range from 2.0 microM to 112 microM. The immobilized alcohol oxidase also showed its direct electrochemistry. The biofunctional nanocomposite provides a new way to not only construct the highly sensitive biosensors but also mimic the catalytic activity of enzyme in the life process. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|