Radioimmunoassay for quantifying the cytokininscis-zeatin andcis-zeatin riboside and its application to xylem sap samples |
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Authors: | C W Parker J Badenoch-Jones D S Letham |
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Institution: | (1) Plant Cell Biology Group, Research School of Biological Sciences, Australian National University, 2601 Canberra, ACT, Australia |
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Abstract: | An antiserum against the cytokinincis-zeatin riboside was raised in rabbits and characterized for use in radioimmunoassays. Cross-reactivity studies demonstrated the specificity of the selected antiserum forcis-zeatin riboside andcis-zeatin in preference to a range of cytokinins and other purines. HPLC systems were developed that separatedcis-zeatin andcis-zeatin riboside from zeatin/dihydrozeatin and zeatin riboside/dihydrozeatin riboside, respectively. These systems enabled the separation of these compounds in xylem sap samples of wheat and oats and their quantification using radioimmunoassay. A TLC system for the separation ofcis-zeatin andcis-zeatin riboside from zeatin/dihydrozeatin and zeatin riboside/dihydrozeatin riboside, respectively, is also described. |
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