Expression and distribution of Gpr119 in the pancreatic islets of mice and rats: predominant localization in pancreatic polypeptide-secreting PP-cells |
| |
Authors: | Sakamoto Yukiko Inoue Hiroshi Kawakami Shuhei Miyawaki Katsuyuki Miyamoto Tatsuro Mizuta Kuniko Itakura Mitsuo |
| |
Affiliation: | Institute for Genome Research, The University of Tokushima, Tokushima, Japan. |
| |
Abstract: | The GPR119 was recently shown to be activated by oleoylethanolamide (OEA), a naturally occurring bioactive lipid with hypophagic and anti-obesity effects. In this study, we have cloned and characterized its murine counterpart, Gpr119. The full-length cDNA contained an open reading frame of 1008bp encoding a 335-amino acid protein. The genomic organization of Gpr119 was unique, having a 3'-untranslated second exon that was also involved in an alternative splicing event. Gene expression analyses confirmed its specific expressions in pancreatic islets and two endocrine cell-lines, MIN6 and alphaTC1. Immunohistochemistry and double-immunofluorescence studies using a specific antibody revealed the predominant Gpr119 localization in pancreatic polypeptide (PP)-cells of islets. No definitive evidence of Gpr119-immunoreactivity in adult beta- or alpha-cells was obtained. The Gpr119 mRNA levels were elevated in islets of obese hyperglycemic db/db mice as compared to control islets, suggesting a possible involvement of this receptor in the development of obesity and diabetes. |
| |
Keywords: | Gpr119 Oleoylethanolamide Pancreatic polypeptide |
本文献已被 ScienceDirect PubMed 等数据库收录! |