Fractionation of chromosomal deoxyribonucleoproteins by partition in two-phase aqueous polymer systems |
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Authors: | G Turner R Hancock |
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Institution: | Department of Cell Biology, Swiss Institute for Experimental Cancer Research, 1011 Lausanne, Switzerland |
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Abstract: | Deoxyribonucleoprotein (DNP)1 prepared by shearing chromatin of mouse cells may be fractionated in 2-phase aqueous Dextran-polyethyleneglycol mixtures. A partial separation of DNPs with different non-histone protein/DNA ratios may be obtained in a single-step partition. Separation of a spectrum of fractions of DNP has been obtained by countercurrent distribution using the same 2-phase polymer system. DNP fractions which bear nascent RNA (representing approximately of the total DNA) may be separated from the major fraction of DNP; they are found in the same region of the distribution pattern as DNP fractions with the highest non-histone protein/DNA ratio. |
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