Optimization of sucrose and inorganic nitrogen concentrations for somatic embryogenesis of birch (Betula pendula Roth.) callus cultures: A statistical approach

The effects of different concentrations of sucrose and inorganic nitrogen on somatic embryogenesis of birch (Betula pendula Roth.) callus cultures were studied using Box-Wilson statistical experimental design. The experimental results were processed by regression analysis, and mathermatical models describing the effects of the selected variables were derived. On the basis of the modeling, maximum embryo production can be achieved with 35 mM total inorganic nitrogen and 20.8 g 1^-1 sucrose in the medium.