Morphological and cytological studies of colchicine-induced tetraploids inTorenia fournieri lind |
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Authors: | S. L. Tandon Kamlesh Bhutani |
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Affiliation: | (1) Department of Botany, University of Delhi, Delhi-6, India |
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Abstract: | The present paper concerns a morphological and cytological comparison between the naturally occurring diploid and the colchicine induced tetraploid ofTorenia fournieri Lind.Treatment of growing points of young seedlings with 0.20% aqueous colchicine for 10 hours on two successive days was most effective in inducing tetraploidy. The induced tetraploids showed the usual gigas characters associated with autotetraploidy. There was a significant reduction in pollen fertility and seed setting of tetraploids as compared to their diploid progenitors.The chromosome numbers of the diploids and the induced tetraploids were determined as n=9 and n=18, respectively. The diploids showed normal meiosis whereas tetraploids were characterized by a high frequency of unequal distribution of chromosomes at anaphase. The induced tetraploids possessed larger flowers. The variability in flower size of the tetraploids offers a good scope for evolving a better horticultural variety of this species. |
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