trans,trans-2,4-decadienal induces mitochondrial dysfunction and oxidative stress |
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Authors: | Carlos A O Sigolo Paolo Di Mascio Alicia J Kowaltowski Camila C M Garcia Marisa H G Medeiros |
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Institution: | 1. Departamento de Bioquímica, Instituto de Química, Universidade de S?o Paulo, Av. Prof. Lineu Prestes 748, CEP 05508-900, S?o Paulo, Brazil
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Abstract: | Lipid peroxidation produces a large number of reactive aldehydes as secondary products. We have previously shown that the
reaction of cytochrome c with trans,trans-2,4-decadienal (DDE), an aldehyde generated as a product of lipid peroxidation in cell membranes, results in the formation
of adducts. Mass spectrometry analysis indicated that His-33, Lys-39, Lys-72 and Lys-100 in cytochrome c were modified by
DDE. In the present work, we investigated the effect of DDE on isolated rat liver mitochondria. DDE (162 μM) treatment increases
the rate of mitochondrial oxygen consumption. Extensive mitochondrial swelling upon treatment with DDE (900 nM–162 μM) was
observed by light scattering and transmission electron microscopy experiments. DDE-induced loss of inner mitochondrial membrane
potentials, monitored by safranin O fluorescence, was also observed. Furthermore, DDE-treated mitochondria showed an increase
in lipid peroxidation, as monitored by MDA formation. These results suggest that reactive aldehydes promote mitochondrial
dysfunction. |
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Keywords: | DDE Mitochondria Lipid peroxidation Mitochondrial swelling |
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