黑龙江省大林姬鼠中汉坦病毒的分离及其S基因序列分析

为了研究黑龙江省大林姬鼠携带汉坦病毒(HV)的分子特征,对黑龙江省大林姬鼠分离株NA33的S基因进行了扩增和序列分析。结果表明,NA33株S基因全长由1 693nt组成,TA含量丰富,编码N蛋白的ORF起始于37nt,终止于1 326nt,编码的蛋白长429aa,符合HTN型编码。与HV参考毒株进行比较,NA33与Amur类汉坦病毒同源性最高,与其它HTN型相对较低,与SEO等同源性更低。N蛋白进化树分析表明,NA33位于Amur类病毒所在支系,并且与俄罗斯远东和吉林大林姬鼠分离株亲缘关系更接近,体现了一定的宿主依赖性和地理簇集性。序列分析发现,NA33的N蛋白具有Amur类汉坦病毒保守的氨基酸位点。黑龙江省大林姬鼠携带Amur类汉坦病毒,是重要的传染源。

Characterization of S Gene of a Strain of Hantavirus Isolated from Apodemus Peninsulae in Heilongjiang Province

In order to study the molecular characterization of the hantavirus isolated from Apodemus peninsulae in Heilongjiang Province,the S gene of a new strain NA33 was amplified,sequenced and analyzed.The results showed that the complete nucleotide sequence of the S gene of NA33 strain was composed of 1 693 nucleotides with TA-rich.The S gene contained one ORF,starting at position 37 and ending at position 1 326,encoding the N protein of 429 amino acid residues,and in line with HTN-based coding.Sequence comparison of the S genes between NA33 and reference hantavirus strains showed that NA33 was more homologous to Amur-like viruses than to the Hantaan(HTN) viruses or the other hantaviruses.Phylogenetic analysis of the amino acid sequence of N proteins showed that NA33 was clustered into the group of Amur-like viruses and was more similar to Far East Russia and Jilin strains isolated from Apodemus peninsulae.The phylogenetic tree indicated a certain degree of host-dependent characteristics and geographical aggregation characteristics of hantanviruses.Furthermore,the amino acid sequence of N protein of NA33 had the conserved amino acid sites of Amur-like viruses.In conclusion,Apodemus peninsulae carried Amur-like viruses in Heilongjiang province and was an important infectious source of hemorrhagic fever with renal syndrome(HFRS).