| 高滴度感染力H5N1亚型禽流感病毒假病毒颗粒的制备 |
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| 引用本文： | 刘熙君,郭志平,沈立萍,王岳,史海燕,张国辉,张寻,毕胜利,赵洪兰.高滴度感染力H5N1亚型禽流感病毒假病毒颗粒的制备[J].病毒学报,2012,28(4):324-329. |
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| 作者姓名： | 刘熙君 郭志平 沈立萍 王岳 史海燕 张国辉 张寻 毕胜利 赵洪兰 |
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| 作者单位： | 温州医学院检验医学院暨生命科学院医学病毒学研究所,温州325035;中国疾病预防控制中心病毒病预防控制所,北京100052;中国疾病预防控制中心病毒病预防控制所,北京100052;北京市第三十五中学,北京100032;中国疾病预防控制中心病毒病预防控制所,北京,100052 |
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| 基金项目： | 传染病防治重大专项(No.2009ZX10004-711) |
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| 摘 要： | 利用一个瞬时共转染系统，将H5N1亚型禽流感病毒的血凝素（Hemagglutinin，HA）蛋白与神经氨酸酶（Neuraminidase，NA）蛋白整合到鼠白血病病毒假病毒颗粒表面，包装成表达HA与NA的假病毒颗粒，通过透射电子显微镜形态学观察、感染滴度分析、血凝试验和中和试验研究其生物学特性。研究获得了高滴度感染力的H5N1假病毒颗粒（H5N1 Pseudotyped particle，H5N1Pp），H5N1Pp的感染力滴度为1E8 Pp/mL，形态、血凝活性及中和特性均与野生H5N1病毒相似，结果为H5N1病毒受体、HA与NA的功能、中和抗体、抗病毒药物开发研究的开展建立了平台。
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| 关 键 词： | 鼠白血病病毒 禽流感病毒 H5N1亚型 假病毒 血凝素 神经氨酸酶 |
Establishing a High-titer Infectious Avian Influenza A(H5N1) Pseudotyped Viral Particle |
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| LIU Xi-jun,GUO Zhi-ping,SHEN Li-ping,WANG Yue,SHI Hai-yan,ZHANG Guo-hui,ZHANG Xun,BI Sheng-li,ZHAO Hong-lan.Establishing a High-titer Infectious Avian Influenza A(H5N1) Pseudotyped Viral Particle[J].Chinese Journal of Virology,2012,28(4):324-329. |
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| Authors: | LIU Xi-jun GUO Zhi-ping SHEN Li-ping WANG Yue SHI Hai-yan ZHANG Guo-hui ZHANG Xun BI Sheng-li ZHAO Hong-lan |
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| Institution: | 1.Institute of Medical Virology,School of Laboratory Medicine and Life Science,Wenzhou Medical College,Wenzhou 325035,China; 2.National Institute for Viral Disease Control and Prevention,China CDC,Beijing 100052,China; 3.Beijing No.35 High School,Beijing 100032,China) |
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| Abstract: | A transient four-plasmid cotransfection system was used to construct avian influenza A(H5N1)pseudotyped viral particle(H5N1Pp) by incorporating hemagglutinin(HA) protein and neuraminidase(NA) protein from H5N1 avian influenza virus onto Murine leukemia virus pseudotyped viral particles,the transmission electron microscopy,infectivity titer assay,hemagglutination assay,neutralization assay of H5N1Pp were studied.We established a pseudotyped H5N1 viral particle at a high titer of 108Pp/mL,the morphology,the hemagglutination activity and neutralization specificity of H5N1Pp is simililar to wild H5N1virus.The research result sets a platform for studying this virus,including its receptors,the functional analysis of HA and NA,neutralizing antibodies and anti-H5N1 drug development. |
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| Keywords: | Murine leukemia virus Avian influenza virus Subtype H5N1 Pseudotyped virus Hemagglutinin Neuraminidase |
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