Cholinergic regulation of neuropeptide Y synthesis and release in human neuroblastoma cells |
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Authors: | Dozio Elena Ruscica Massimiliano Feltrin Daniel Motta Marcella Magni Paolo |
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Institution: | Istituto di Endocrinologia, Center of Excellence on Neurodegenerative Diseases and Center for Endocrinological Oncology, University of Milan, Milano, Italy. |
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Abstract: | The biosynthesis and release of neuropeptide Y (NPY) is regulated by several factors. Here, the effect of the muscarinic agonist carbachol on NPY biosynthesis and release was analyzed utilizing the SH-SY5Y human neuroblastoma cell line. We observed that: (a) carbachol moderately increased the post-translational cleavage of proNPY to NPY; (b) carbachol treatment stimulated NPY accumulation into the medium in a time- and dose-related manner; (c) protein kinase C activation is involved in carbachol-mediated NPY synthesis/release (>6h). In conclusion, the present observations support the hypothesis that muscarinic receptor activation regulates the biosynthesis and secretion of NPY. |
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