柽柳金属硫蛋白基因的克隆及序列分析

用木麻黄(Casuarina glauca)的金属硫蛋白基因(metallothionein 1)氨基酸序列对柽柳ESTs序列本地数据库进行tBlastn检索,获得了柽柳金属硫蛋白基因全长cDNA序列，去除polyA后该基因全长366 bp，其中5′非翻译区97 bp，3′非翻译区59 bp，开放读码框(ORF)长210 bp，编码70 个氨基酸组成的多肽，蛋白分子量为6.793 kD，理论等电点为4.99，含10个Cys，集中分布在肽链的N端和C端。BlastP同源性分析表明该基因与花生同源性最高，与小豆同源性最低。该基因的EST序列在GenBank登录(登录号：CV792539)。

Cloning and Analysis of Metallothionein Gene from Tamarix androssowii

The amino acid sequence of metallothionein gene from Casuarina glauca was subjected to local tBlastn searching against the ESTs local datebase of Tamarix androssowii. The full length cDNA encoding metallothionein (MT) gene was obtained. The MT gene was 366 bp in length without its polyA sequence, including 97 bp of 5′untranslated region and 59 bp of 3′untranslated region. It had an open reading frame(ORF) of 210 bp, encoding 70 amino acid residues of which 10 Cys residues distributed at protein N terminal and C terminal. The protein molecular weight was 6.793 kD and theoretical pI was 4.99. The BlastP analysis revealed that amino acids sequence of MT gene from Tamarix androssowii shared the highest similarity with Arachis hypogaea and the lowest similarty with Vigna angularis. The EST sequence of MT gene was accepted by GenBank (accession numbers: CV792539).