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Characterization of Nasal Potential Difference in cftr Knockout and F508del-CFTR Mice |
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| Authors: | Emilie Lyne Saussereau Delphine Roussel Siradiou Diallo Laurent Debarbieux Aleksander Edelman Isabelle Sermet-Gaudelus |
| Institution: | 1. Institut Pasteur, Molecular Biology of the Gene in Extremophiles Unit, Department of Microbiology, Paris, France.; 2. INSERM, U 845, Université Paris Descartes, Faculté de Médecine Necker Enfants-Malades, Paris, France.; 3. Université Pierre et Marie Curie, Cellule Pasteur UPMC, Paris, France.; University of Alabama-Birmingham, United States Of America, |
| Abstract: | BackgroundTreatments designed to correct cystic fibrosis transmembrane conductance regulator (CFTR) defects must first be evaluated in preclinical experiments in the mouse model of cystic fibrosis (CF). Mice nasal mucosa mimics the bioelectric defect seen in humans. The use of nasal potential difference (VTE) to assess ionic transport is a powerful test evaluating the restoration of CFTR function. Nasal VTE in CF mice must be well characterized for correct interpretation.MethodsWe performed VTE measurements in large-scale studies of two mouse models of CF—B6;129 cftr knockout and FVB F508del-CFTR—and their respective wild-type (WT) littermates. We assessed the repeatability of the test for cftr knockout mice and defined cutoff points distinguishing between WT and F508del-CFTR mice.ResultsWe determined the typical VTE values for CF and WT mice and demonstrated the existence of residual CFTR activity in F508del-CFTR mice. We characterized intra-animal variability in B6;129 mice and defined the cutoff points for F508del-CFTR chloride secretion rescue. Hyperpolarization of more than -2.15 mV after perfusion with a low-concentration Cl- solution was considered to indicate a normal response.ConclusionsThese data will make it possible to interpret changes in nasal VTE in mouse models of CF, in future preclinical studies. |
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