Upgrading Fungal Gene Expression on Demand: Improved Systems for Doxycycline-Dependent Silencing in Aspergillus fumigatus |
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| Authors: | Christoph Helmschrott Anna Sasse Sweta Samantaray Sven Krappmann Johannes Wagener |
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| Institution: | aMax von Pettenkofer-Institut, Ludwig-Maximilians-Universität, Munich, Germany;bResearch Center for Infectious Diseases, Julius-Maximilians-University Würzburg, Würzburg, Germany;cMikrobiologisches Institut—Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen and Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany |
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| Abstract: | Conditional gene expression is key for functional studies in any given microorganism. To allow tight regulation in the pathogenic mold Aspergillus fumigatus, improved versions of the doxycycline-dependent Tet-On system were generated by replacing functional elements of the precursor module, thereby circumventing the former problem of leakiness due to intramolecular recombination. |
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