A new principle of cell cultivation: No air-liquid interface but gas exchange across a synthetic membrane |
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Authors: | J Larsen Jytte R Nilsson |
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Institution: | (1) Institute of Cell Biology and Anatomy, The Zoological Institutes, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen Ø, Denmark |
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Abstract: | Summary A completely liquid-filled growth chamber for axenic cultures ofTetrahymena pyriformis is described; gas exchange is ensured across a synthetic membrane. The chamber may be incorporated into a continuous flow system with inoculation and removal of cell samples under sterile conditions. Initially, the generation time of the cells was slightly prolonged, about 10%, but after some cell doublings decreased to 5%. The capacity of the cells to form food vacuoles (endocytosis) was unaltered during growth in the chamber. The synthetic membrane was highly permeable to O2 and CO2; however, cells grown in the chamber contained small refractive granules. The culture chamber permits the culture volume to be varied and it may be used for other protozoa, bacteria, and even tissue culture cells. |
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Keywords: | Liquid-filled culture chamber Gas exchange across synthetic membrane Continuous flow system Growth characteristics Tetrahymena pyriformis |
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