Increased oxidative stress alters nucleosides metabolite levels in sickle cell anemia |
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| Authors: | Lívia Gelain Castilhos Juliana Sorraila de Oliveira Stephen Adeniyi Adefegha Luana Pereira Magni Pedro Henrique Doleski Fatima Husein Abdalla |
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| Affiliation: | 1. Programa de Pós-Gradua??o em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria-RS, Brazilliviagelain@gmail.com;3. Programa de Pós-Gradua??o em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria-RS, Brazil;4. Department of Biochemistry, Federal University of Technology, Akure, Nigeria |
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| Abstract: | Objectives: This study was conducted to assess the markers of oxidative stress, myeloperoxidase (MPO), acetylcholinesterase (AChE) and xanthine oxidase (XO) activities as well as the levels of nucleotide metabolites in sickle cell anemia (SCA) patients.Methods: Fifteen SCA treated patients and 30 health subjects (control group) were selected. The markers of oxidative stress (levels of reactive oxygen species (ROS), plasma proteins, carbonyl content, lipid peroxidation (TBARS), total thiols (T-SH), glutathione and catalase activity), MPO, AChE and XO activities as well as the levels of nucleotide metabolites were measured in SCA patients.Results: ROS, thiobarbituric acid-reactive substances (TBARS) and T-SH levels as well as the activities of catalase and MPO were significantly increased while glutathione level was reduced in SCA patients. Furthermore, a significant (P?P?P?P?Discussion: The altered parameters in SCA patients suggest that the generation and impairment of oxidative stress in this disease as well as antioxidant markers are contributory factors towards cellular redox homeostasis and alteration of purine metabolites. |
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| Keywords: | Sickle cell anemia oxidative stress enzymes reactive oxygen species lipid peroxidation xanthine hypoxanthine uric acid |
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