Preparation and regeneration of protoplasts of three fungi of Boletaceae |
| |
Authors: | Masatake Ohmasa Shinya Kikuchi Kumiko Kawakami |
| |
Affiliation: | (1) Laboratory of Applied Mushroom Sciences, Faculty of Agriculture, Shinshu University, 8304 Minamiminowa-mura, Kamiina-gun, 399 Nagano, Japan;(2) Present address: Nihonshokuken Co. Ltd., 794 Imabari-shi, Ehime, Japan;(3) Present address: Eastern Co. Ltd., 391 Chino-shi, Nagano, Japan |
| |
Abstract: | Protoplasts of three fungi of Boletaceae,Suillus luteus, S. grevillei, andBoletinus cavipes, were prepared with yields of 45, 8.0, and 1.8×107/g fresh mycelia under the optimal conditions, respectively. Nucleate protoplasts accounted for 42% of the whole preparation ofS. luteus and 32% of that ofS. grevillei, and 21% of the nucleate protoplasts ofS. luteus and 35% of those ofS. grevillei possesed two nuclei. Regeneration efficiency of protoplasts was 0.4% forS. luteus and 0.05% forS. grevillei. The regeneration ofB. cavipes protoplasts was also confirmed. Optimal conditions for regeneration were determined. Addition of gellan gum instead of agar to the medium and activated charcoal treatment of agar medium increased the regeneration efficiency significantly. |
| |
Keywords: | Boletinus cavipes protoplast formation protoplast regeneration Suillus grevillei Suillus luteus |
本文献已被 ScienceDirect SpringerLink 等数据库收录! |
|