An in vivo analysis of the energetics of aldose oxidation by Acinetobacter calcoaceticus |
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| Authors: | B. J. van Schie R. J. Rouwenhorst J. A. M. de Bont J. P. van Dijken J. G. Kuenen |
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| Affiliation: | (1) Laboratory of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67a, 2628 BC Delft, The Netherlands;(2) Present address: Unilever Research Laboratory, Department of Bioprocessing, Olivier van Noordlaan 120, 3133 AT Vlaardingen, The Netherlands;(3) Present address: Dept of Microbiology, Agricultural University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands |
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| Abstract: | Summary A continuous culture study was made of the energetics of oxidation of various aldose sugars by Acinetobacter calcoaceticus LMD 79.41. The consumption of aldoses during carbon- and energy-limited growth of the organism on mixtures of acetate and an aldose was independent of the pH of the culture. Acid production, however, was strongly dependent on this parameter. It is shown that aldose consumption without concurrent acid production is due to formation of the corresponding lactone, the hydrolysis of which is pH-dependent. The cell yield of A. calcoaceticus on mixtures of acetate and glucose or xylose was much higher than during growth on acetate alone. This increase in cell yield was, however, dependent on the pH of the culture. Only at pH values which permitted a high rate of lactone hydrolysis an enhancement of the cell yield was observed. These results suggest that lactone hydrolysis has an important bearing on the efficiency of periplasmic oxidation of aldoses in bacteria. |
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