Use of microarray analysis to study gene expression in the avian epiphyseal growth plate |
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| Authors: | M Horvat-Gordon CA Praul R Ramachandran PA Bartell RM Leach |
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| Institution: | 1. Department of Medicine, Surgery and Neuroscience, Rheumatology Unit, University of Siena, Policlinico Le Scotte, Viale Bracci 1, 53100 Siena, Italy;2. Servicio de Reumatología, Instituto de investigación Biomedicas A Coruña (INIBIC), Hospital Universitario A Coruña (CHAUC) Sergas, Universidade da Coruña, A Coruña, Spain;3. Centro de investigación biomédica en Red, Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Madrid, Spain;4. Department of Chemistry and Drug Technologies, University “La Sapienza” Rome, P.le Aldo Moro, 5, 00185 Rome, Italy;5. Rottapharm Biotech, Via Valosa di Sopra 6, 20126 Monza, Italy;6. Department of Biotechnology, Chemistry and Pharmacy, DoE, University of Siena, Via A. Moro, 2, 53100 Siena, Italy;1. Rheumatology Division, ProteoRed/ISCIII Proteomics Group, INIBIC – Hospital Universitario de A Coruña, 15006 A Coruña, Spain;2. CIBER-BBN Instituto de Salud Carlos III, INIBIC-CHUAC, A Coruña, Spain;3. ProteoRed-ISCIII, Centro Nacional de Biotecnología - CSIC, UAM Campus Cantoblanco, Darwin, 3, 28049 Madrid, Spain;4. ProteoRed-ISCIII, Functional Proteomics, Department of Cellular and Molecular Medicine, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain;5. ProteoRed-ISCIII, Center for Applied Medical Research (CIMA), University of Navarra, Pío XII, 55; Ed. CIMA, 31008 Pamplona, Spain;6. ProteoRed-ISCIII, Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza de Ramón y Cajal, 28040 Madrid, Spain;7. ProteoRed-ISCIII, Department of Immunology, IIS-Fundacion Jimenez Diaz, Madrid, Spain;8. RIER-RED de Inflamación y Enfermedades Reumáticas, INIBIC-CHUAC, A Coruña, Spain |
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| Abstract: | Longitudinal bone growth depends upon the execution of an intricate series of cellular activities by epiphyseal growth plate chondrocytes. In order to better understand these coordinated events, microarray analysis was used to compare gene expression in chondrocytes isolated from the proliferative and hypertrophic zones of the avian growth plate. RT-PCR was used to confirm the identity of a select number of genes. The expression of 745 genes was found to differ 3-fold or greater at the 0.05 level of probability. Transferrin was the most highly up-regulated (321-fold) gene associated with chondrocyte hypertrophy. Immunohistochemistry localized this peptide adjacent to the penetrating blood vessels in the growth plate of 3-week-old chicks. Fibulin, OC-116, DMP-1 and PHEX were among the expanded number of genes associated with extracellular matrix metabolism. The presence of NELL2, ATOH8 and PLEXIN suggests a neuronal involvement in growth plate physiology. In addition, the expression of a large number of genes associated with angiogenesis and cellular stress was up-regulated. These processes are important to the physiology and survival of chondrocytes in the unique and stressful environment of the epiphyseal growth plate. |
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