昆虫细胞无血清培养

血清是细胞培养基常用的添加剂。目前应用最广泛的动物血清是胎牛血清。随着现代细胞生物学在细胞和组织培养方面的进步以及细胞培养方法的标准化，人们更多的注意到了胎牛血清收集中的伦理道德问题。按照3Rs的原则，科学家希望通过减少血清用量和开发使用血清替代物的方法来减少每年对血清的需求；另外由于血清成分并不明确，考虑到改进细胞和组织培养方法的要求，很多无血清细胞培养基陆续开发成功，成为替代胎牛血清的一个比较科学的方法。

Serum-free Culture of Insect Cells

Serum is commonly used as a supplement to cell culture medium. The most widely used animal serum is fetal bovine serum, FBS. With the improvement of modern cell biology in cell and tissue culture and the standardization of cell culture protocols, considerable ethical concerns were fixed recently on the harvest and collection of FBS. Thus, in order to decrease the annual need of animal serum in terms of the 3Rs, any reduction in the use or partial replacement of serum is expected. In addition, because of serum being an ill-defined component and in terms of an improvement of cell and tissue culture methodology, many serum-free cell culture medium were developed successfully and were well accepted as an alternative to the use of FBS in cell and tissue culture.