Some Factors Affecting the Anthocyanin Formation by Populus Cells in Suspension Culture |
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Authors: | Takashi Matsumoto Koh Nishida Masao Noguchi Einosuke Tamaki |
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Affiliation: | The Central Research Institute, Japan Monopoly Corporation, 6, Umegaoka, Midori-ku, Yokohama |
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Abstract: | In order to elucidate the mechanism of anthocyanin formation by Populus cells in suspension culture, the favourable conditions for anthocyanin formation were investigated. The influence of some factors affecting the anthocyanin formation, i.e. light, sucrose and riboflavin etc. were also examined. Light irradiation and high sucrose concentrations brought about a marked increase of PAL activity, which increased rapidly at the lag phase preceding the anthocyanin formation. The effect of blue light on anthocyanin formation was markedly superior to other kinds of monochromatic light (green and red) or white light. Riboflavin was effective only under light exposure. It was inferred that light, sucrose, riboflavin and PAL activity etc. were closely related with the anthocyanin formation. Especially, light and sucrose cooperated in the increase of PAL activity which was a key enzyme in the biosynthesis of anthocyanin. |
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Keywords: | Arabidopsis thaliana 26S proteasome Zn deficiency oxidative stress |
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