Minor Components of Rat Liver Ribosomal RNA as Possible Intermediates in the Degradation of 28S RNA in Vivo |
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Authors: | Tadashi Inoue Masamichi Takagi Yasukiyo Umemura Hikoyuki Yamaguchi |
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Affiliation: | 1. Laboratory of Radiation Genetics, Faculty of Agriculture, University of Tokyo, Tokyo;2. National Institute of Animal Health, Kodaira, Tokyo |
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Abstract: | The minor RNA components in large ribosomal subunits of rat liver were analyzed by gel electrophoresis. Quantitative analysis showed that these minor components, whose apparent molecular weights ranged from 8.48 × 105 to 11.4 × 105, represented 10 to 13% of the total high-molecular-weight ribosomal RNA.It was elucidated that they were not artifacts arose during the cell homogenization, sub-cellular fractionation, RNA preparation and gel electrophoresis.Labeling experiment in vivo showed that they were preferentially present in “old” ribosomes. It was predicted that these minor components were the intermediates in the degradation of 28S ribosomal RNA in vivo.In the regenerating liver, where the degradation of proteins was reported to be blocked, the relative amount of the minor RNA components was decreased to about a half of that of normal liver. There was, however, no increase in their relative amount in the long-term starved rat liver, where RNA degradation should be going very rapidly. |
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Keywords: | activity glycine metalloproteinase site-directed mutagenesis thermolysin |
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