The Isolation and Characterization of the Two Macromolecular Antitumor Agents from Streptomyces |
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Authors: | Shigeharu Inouye |
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Institution: | Research Laboratories of Meiji Seika Kaisha, Ltd., Yokohama |
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Abstract: | Purification of the two antitumor macromolecules, A216 and A280 substances, from culture filtrates of Streptomyces is achieved by chromatography using ion-exchanged celluloses. The purified macromolecules appeared homogeneous are characterized as a protein from the chemical and biological properties by paper electrophoresis, paper chromatography and ultracentrifuge.The simple method for approximation of molecular weight of a protein from distribution coefficient on gel filtration is proposed. The molecular weights of both macromolecules given by gel filtration are near to those given by ultracentrifugal analysis. |
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