Systematic Determination of Various Forms of Choline in Plants |
| |
| Authors: | Isao Toyosawa Uzo Nishimoto |
| |
| Institution: | Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture, Osaka |
| |
| Abstract: | A rapid and systematic method is described for the determination of lipid-choline, free choline and two water-soluble bound cholines in plant tissues. These substances are extracted with isopropanol and methanol, avoiding the enzymatic degradation. The extract is fractionated into lipid-choline and water-soluble choline by applying the solvent-partitioning method of Folch et al. The latter fraction is passed through Amberlite IRC-50 column, and separated into free choline and bound-choline fractions. Two forms of acid-labile choline are determined after hydrolyzing the bound-choline fraction with hydrochloric acid. The method was applied to the determination of various forms of choline in several plants, and lipid-choline and free choline were found to be major components. |
| |
| Keywords: | |
|
|
