Purification of Cytochrome a of an L-Glutamate-producing Microorganism,Brevibacterium thiogenitalis |
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Authors: | Yoshio Sugiyama Kazuaki Kitano Toshihiko Kanzaki |
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Affiliation: | Takasago Plant, Takeda Chemical Industries, Ltd., Takasago, Hyogo, Japan |
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Abstract: | The respiratory chain system of Brev. thiogenitalis grown in the presence of copper ions contained cytochromes a, b and c. The cytochrome a was solubilized and purified from the cell-free extracts by means of Triton X-100 and cholate extraction, and DEAE-cellulose chromatography. It was purified about 130-fold from the cell-free extracts and was free from other cytochromes, The purified preparation contained 1.4 mμatom copper and 1.9 mμatom iron per mμmole heme a, respectively, and approximately 5 mμmoles heme a per mg protein. |
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Keywords: | 4-hydroxyisoleucine aldolase transaminase Arthrobacter simplex antidiabetics |
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