Deacetylcephalosporin C Formation by Cephalosporin C Acetylhydrolase Induced in a Cephalosporium acermonium Mutant |
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Authors: | Yukio Fujisawa ShirafujiHideo Shirafuji Toshihiko Kanzaki |
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Institution: | Microbiological Research Laboratories, Central Research Division, Takeda Chemical Industries, Ltd., Yodogawa-ku, Osaka 532, Japan |
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Abstract: | Cephalosporin C acetyl-hydrolase, which had not yet been found in Cephalosporium acremonium cultures, was partially purified from the culture fluid of the mutant No. 81 by ammonium sulfate fractionation, dialysis and DEAE-cellulose column chromatography. The optimum pH and temperature of the enzyme reaction were found to be about 8.0 and 50°C, respectively. The enzyme activity was hardly affected by Mg2+, Mn2+, Zn2+, Co2+, Ni2+, Na+, K+, EDTA, PCMB and 2,4-dinitrophenol, but markedly inhibited by diisopropylfluoro-phosphate at 1 mm. The product formed from cephalosporin C by the enzyme reaction was proved to be deacetylcephalosporin C by physical and chemical analyses and chromatographic behaviors. |
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Keywords: | Sparassis crispa (hanabiratake) β-glucan wound healing |
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