Studies on dl-Alanine Formation by Thermophilic Bacteria |
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Authors: | Takashi Matsumoto Toshimasa Yano Koichi Yamada |
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Affiliation: | Department of Agricultural Chemistry, The University of Tokyo, Tokyo |
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Abstract: | During the course of the screening of thermophilic microorganisms, several strains were found to accumulate amino-acids. Of those strains that were isolated from feces source with Bennett medium, one strain was found to produce a large amount of an amino acid. This amino acid was isolated in crystalline form and identified as dl-alanine by IR absorption spectrum, specific rotatory power and elementary analysis. The taxonomic studies were carried out and this strain was identified as Bacillus coagulans. The strain B. coagulans B9-17 produced dl-alanine as much as 1000 mg/l after 24 hours at 50°C in shake culture.The yield of dl-alanine was increased up to 16.5 g/l with some improvements. |
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