Automated Measurement of L-Glutamate in Soy Sauce Using L-Glutamate Dehydrogenase

An automated method for rapid and convenient measurement of L-glutamate has been developed by using a discrete analyzer, EEL Auto Chemist. It is based on the colorimetric measurement of NADH produced on a mole-mole basis by enzymatic dehydrogenation of L-glutamate using L-glutamate dehydrogenase from bovine liver. The values of L-glutamate obtained by this method were well agreed with those obtained by the routine Waruburg mano-metric method using L-glutamate decarboxylase from Escherichia coli.