Extracellular Accumulation of DNA by Hydrocarbon-utilizing Bacteria |
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Authors: | Fusao Tomita Takeo Suzuki |
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Affiliation: | Tokyo Research Laboratory, Kyowa Hakko Kogyo Co., Ltd., Machida, Tokyo |
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Abstract: | Some hydrocarbon-utilizing bacteria grown on n-paraffin as the sole source of carbon accumulated extracellularly a considerable amount of DNA (0.1 g to 0.6 g per liter) which was free from intact cells and slime materials. This was particularly noted when the strains belonging to Pseudomonad were employed. n-Paraffin was a preferable carbon source for the accumulation of DNA by Pseudomonad, while a strain of Arthrobacter accumulated DNA by growing it on glucose.The DNA thus accumulated was easily isolated and purified free from other cellular components. The purified DNA was highly polymerized (above 4 × l06 daltons), having the same base composition as the cellular DNA. |
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Keywords: | Alteromonas sp. bifunctional alginate lyase guluronate mannuronate subsite structure |
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