Poduction of Biotin by Microbial Transformation of dl-cis-Tetrahydro-2-oxo-4-n-pentyl-thieno-(3,4-d)-imidazoline (dl-TOPTI) |
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Authors: | Shigeo Ogino Shigehisa Fujimoto Yasuhiko Aoki |
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Institution: | Research Department, Pharmaceutical Division of Sumitomo Chemical Co., Ltd., Takarazuka, Hyogo Prefecture, Japan |
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Abstract: | The conditions for biotin production were investigated. Urea was a more effective nitrogen source than ammonium chloride and ammonium sulfate. About 60% conversion from dl-cis-tetrahydro-2-oxo-4-n-pentyl-thieno-(3,4-d)-imidazoline (dl-TOPTI) to biotinol and biotin occurred using Corynebacterium sp. B–321. Strain M–6318 which derived from B–321 as a mutant incapable of assimilating n-alkane produced large amounts of dl-biotin from dl-TOPTI. The inability of the microbe to assimilate n-alkane resulted in repression of biotin degradation. Maximum conversion (80%) was obtained by growing cultures of strain M–6318 in the constant presence of n-paraffin. |
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Keywords: | α-glucosylhesperidin cold sensitivity peripheral body temperature autonomic nervous system heart-rate variability |
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