Molecular Weight Determination of Component Polypeptides of Glutenin after Fractionation by Gel Filtration |
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Authors: | Zen-ichiro Hamauzu Yukio Kamazuka Hirokazu Kanazawa Daizo Yonezawa |
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Affiliation: | Laboratory of Food Chemistry, College of Agriculture, University of Osaka Prefecture, Sakai, Japan |
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Abstract: | Reduced and cyanoethylated glutenin was fractionated into three fractions (F I, F II and F III) by gel filtration on Sephadex G–100 in 0.1 m acetic acid. The molecular weight determination was made with these three fractions by sedimentation equilibrium in 6.5 m guanidine hydrochloride containing 0.01 m acetic acid. The molecular weight obtained was 44,000 for F II, and 32,000 for F III. F I showed a distribution of molecular weight due to the aggregation. The average molecular weight of F I was 52,000, being 27,000 at the meniscus and 98,000 at the bottom. The estimation of molecular weight by SDS–PAGE* gave overestimated values for glutenin polypeptides, as was already reported for gliadin. |
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Keywords: | antioxidant antimelanogenic chestnut flower tyrosinase melanin |
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