Studies on ATP Deaminase |
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| Authors: | Shiau-Ta Chung Mitsutoshi Hamano Kȏ Aida Teijiro Uemura |
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| Institution: | Institute of Applied Microbiology, University of Tokyo |
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| Abstract: | Water insoluble ATP deaminase was prepared by binding native ATP deaminase to DEAE-cellulose and several enzymatic properties of bound ATP deaminase were compared with those of free deaminase. The optimum pH of the former was 3.0 and shifted toward acid side by about 2 pH units compared with that of the latter. The activity of bound deaminase to ATP corresponded to about one fourth of free deaminase at pH 3.0. The possibility of continuous preparation of IMP from AMP by use of bound deaminase was suggested. |
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