Isolation and Identification of a Microorganism which Produces Non Streptomyces Pepsin Inhibitor and N-Diazoacetyl-dl-norleucine Methylester Sensitive Acid Proteases |
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Authors: | Sawao Murao Kōhei Oda Yoshiyuki Matsushita |
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Institution: | Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture, Sakai, Japan |
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Abstract: | Non pepsin inhibitor (S–PI) and diazoacetyl-dl-norleucine methylester (DAN) sensitive acid proteases producing microorganism was isolated from farm soil of Osaka Prefecture.The isolated strain was identified as Scytalidium lignicolum M–133. When it was aerobically grown on a medium consisting of glucose 5%, meat extract 1.5%, yeast extract 0.1%, KH2PO4 0.2%, MgSO4·7H2O 0.05% at pH 3.5 and 25°C, the strain produced two acid proteases, A and B, in the culture broth.The acid proteases A and B were not at all inactivated by S–PI and DAN. These acid proteases were expected to be a new type of acid protease from the viewpoint of the active site. |
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Keywords: | carbonyl reductase Cylindrocarpon sclerotigenum IFO 31855 (S)-ethyl 4-chloro-3-hydroxybutanoate |
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