Infrared Spectroscopy of Some Mannans |
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Authors: | Koji Kato Masami Nitta Takashi Mizuno |
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Affiliation: | Department of Agricultural Chemistry, Faculty of Agriculture, Shizuoka University, Iwata-shi, Shizuoka-ken |
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Abstract: | Bacterial production of 4-hydroxy(3,4-d)pyrazolopyrirnidine riboside (AP–R) was studied. Seven among 73 tested strains were found to produce AP–R through N-ribosyl transfer reaction between uridine and 4-hydroxy(3,4-d)pyrazolopyrimidine (allopurinol). AP–R was produced by the cell-free extract of Erwinia carotovora and was isolated in crystals from the reaction mixture. The crystalline AP–R was characterized by spectroscopic data and was confirmed to be β-1-ribosyl allopurinol. AP–R could not substitute for inosine to support the growth of a nonexacting purine base-requiring mutant of Sarcina lutea. |
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Keywords: | Citrus unshiu cold stress dehydrin family cDNA cloning |
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