Protein-like Activator for n-Alkane Oxidation by Pseudomonas aeruginosa S7B1 |
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Authors: | Kenichi Hisatsuka Tadaatsu Nakahara Koichi Yamada |
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Institution: | Department of Agricultural Chemistry, The University of Tokyo |
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Abstract: | During the study of the n-alkane oxidation by Pseudomonas aeruginosa S7B1, a nondialysable activator for n-alkane oxidation was discovered in the culture broth of the strain. The activator was purified, as judged by cellulose acetate membrane electrophoresis, by ammonium sulfate precipitation and chromatography on DEAE-Sephadex, CM-Sephadex and Sephadex G-75 columns.The purified activator, which was positive in protein color reactions, remarkable stimulated only the oxidation of n-hexadecane, though it was not observed in case of palmitic acid or glucose oxidation.Co-operative action between the activator and rhamnolipid, which had been isolated as a growth stimulant of P. aeruginosa S7B1 on n-hexadecane by the authors, was observed not only in the oxidation of n-hexadecane but also in the growth on n-hexadecane. |
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