Polarographic Determination of Respiratory Activity with Clark Oxygen Electrode |
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| Authors: | Hisateru Mitsuda Kazuo Murakami Fumio Kawai |
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| Affiliation: | Laboratory of Nutritional Chemistry, Faculty of Agriculture, Kyoto University, Kyoto |
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| Abstract: | The authors have developed a polarographic technique in which Clark oxygen electrode (stationary platinum electrode covered with a polyethylene membrane) is employed for the determination of respiratory activity. The procedure of the operation is very simple, yet it was highly sensitive to the changes of the oxygen concentration in the solution, and the value of current was proportional to the oxygen concentration. The respiratory activity and control of rat liver mitochondria could be determined satisfactorily by this method. |
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