The X-ray Diffraction Pattern of Spores of Bacillus subtilis

ORD and CD of d(+) pantothenic acid, d(+) pantothenyl alcohol and d(?) pantolactone were studied. The acid and the alcohol gave positive Cotton effects with a peak at 227 and 225 mμ, respectively, and the lactone gave a negative Cotton effect with trough at 233 mμ. They gave CD maxima at 214, 213 and 219 mµ corresponding to the inflection points of their ORD curves.

The concentrations were found to be linear to the rotation angles and the possibility of the application to quantitative analysis of the ORD was cited. The ORD showed the quantitative formation of the lactone by acid treatment of the vitamins without any racemization and hence the determination via lactone was suggested.